And so we can take this radioactive DNA probe and we can put it into both of these samples and under the right conditions. ![]() What we can do is we can use a radioactive DNA probe, which is going to be a single stranded DNA molecule, as you say here, which is going to be radioactively labelled. And so suppose that we want to identify which of these samples has a specific DNA sequence of interest. And both of these samples contained D N A. We have sample a over in the left chest tube and sample B over here in the right test tube. That is complementary to it in different samples and so noticing this image Over here on the left hand side, we have two different test tubes with different samples. And so here, in our example saying radioactive probes are used to identify D N A. And so if we take a look at our image down below, we can get a better understanding of these radioactive probes. And so the DNA probe, which is going to be single, stranded and radioactive when it is bound to the specific sequence of interest we can detect the presence of a specific sequence of interest by detecting the presence of the radioactive probe. And so DNA probes are going to be single stranded DNA molecules that are going to be complementary to a specific sequence of interest. And so a probe or probes are really just radioactively labelled molecules that are visualized using radioactive detection. ![]() And so it's important to know is that after cloning a gene, the DNA that was cloned can actually be used as a probe to detect the same sequence and an unknown DNA sample. ![]() And in our next lesson video, we'll talk more about the Southern blotting technique. And so in this video we're going to focus on radioactive probes. But before we talk about the actual Southern blotting technique, it's actually first helpful to talk about radioactive probes. In this video, we're going to begin our lesson on a technique that's called Southern blotting.
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